Upcoming Webinars

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Measuring the Stain Index with FCS Express
Wednesday Sept 5th 1 pm Eastern Time (60 Minutes)
Presented by Juan Vazquez

In this webinar we will teach you how to easily calculate Stain Index in FCS Express using integrated spreadsheets and graphing tools.

In flow cytometry, experiment and panel design has become integral. As flow cytometry technology has evolved and more fluorochromes become available to researchers, a method to help optimize experimental design was needed. The Stain Index is a method to help determine the relative intensity for a fluorochrome on a given instrument and is calculated for a fluorescent parameter by subtracting the Median of the negative population from the Median of the positive population, then dividing that quantity by the variance of the negative population, multiplied by 2.

By determining the relative brightness of a series of fluorochromes, a flow cytometry user can simplify the process by which they design an experiment and use this metric to match fluorochromes with a higher Stain Index with antigens that are of a lower density or a lower affinity.

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